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. 2023 Apr 25;21(5):264. doi: 10.3390/md21050264

Figure 3.

Figure 3

Solution competition between heparin and heparin Ib glycans. (A) SPR sensorgrams of the MPXV A35 protein–heparin interaction competing with different Ib glycans. The concentration of the MPXV A35 protein was 250 nM mixed with 100 µg/mL of different Ib glycans. (B) Bar graphs (based on triplicate experiments with standard deviation) of normalized MPXV A35 protein binding preference to surface heparin by competing with different Ib glycans. (C) SPR sensorgrams of the MPXV A29 protein–heparin interaction competing with different Ib glycans. The concentration of the MPXV A29 protein was 250 nM mixed with 100 µg/mL of different Ib glycans. (D) Bar graphs (based on triplicate experiments with standard deviations) of the normalized MPXV A29 protein binding preference to surface heparin by competing with different Ib glycans. Statistical analysis was performed using an unpaired two-tailed t-test (*: p ≤ 0.05 compared with the control, **: p ≤ 0.01 compared with the control).