Skip to main content
. 2023 Apr 25;21(5):264. doi: 10.3390/md21050264

Figure 5.

Figure 5

Solution competition between heparin and LvSF and PpFucCS glycans. (A) SPR sensorgrams of the MPXV A35 protein–heparin interaction competition with different LvSF and PpFucCS glycans. The concentration of the MPXV A35 protein was 250 nM mixed with 100 µg/mL of different LvSF and PpFucCS glycans. (B) Bar graphs (based on triplicate experiments with standard deviations) of the normalized MPXV A35 protein binding preference to surface heparin by competing with different LvSF and PpFucCS glycans. (C) SPR sensorgrams of the MPXV A29 protein–heparin interaction competing with different LvSF and PpFucCS glycans. The concentration of the MPXV A29 protein was 250 nM mixed with 100 µg/mL of different LvSF and PpFucCS glycans. (D) Bar graphs (based on triplicate experiments with standard deviations) of the normalized MPXV A29 protein binding preference to surface heparin by competing with different LvSF and PpFucCS glycans. Statistical analysis was performed using an unpaired two-tailed t-test (*: p ≤ 0.05 compared with the control).