Table 2.

Summary of solution competition between heparin and eight marine-derived glycans binding to MPXV proteins ^a.

	Control b	Heparin	IbSF	desIbSF	IbFucCS	desIbFucCS	HfSF	HfFucCS	PpFucCS	LvSF
Normalized A29 protein binding (%)	100	43.3 *	5.0 **	75.4 *	7.6 *	66.8 *	8.5 **	13.9 *	13.0 *	23.6 *
Normalized A35 protein binding (%)	100	27.7 *	24.3 *	60.4 *	20.1 *	63.9 *	23.9 *	23.0 *	19.0 *	16.9 *

^a SPR sensorgrams of MPXV A29/A35 protein–heparin interaction competing with eight different marine-derived glycans. The concentration of proteins was 250 nM mixed with 100 µg/mL of different glycans. ^b The control had the same concentration of A29/A35 protein (125 nM) without any mixed glycans. Statistical analysis was performed using an unpaired two-tailed t-test (*: p ≤ 0.05 compared with the control, **: p ≤ 0.01 compared with the control).