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Effects of different polar extracts of maca on TM3 cell activity. (A) TM3 cell proliferation was measured using MTT in the presence of different concentrations of NBH. (B) A fluorescent microscopic image of DAPI-stained TM3 cells after NBH treatment (×100). (C) Testosterone was detected in TM3 cells via immunofluorescence assays. (D) # p < 0.05, ## p < 0.01 and ### p < 0.001 compared to the Sham group; * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the Model (H2O2) group.
