Skip to main content
. 2023 Apr 30;15(5):316. doi: 10.3390/toxins15050316

Figure 1.

Figure 1

Binding of scFv to BoNT/Gi and native BoNT/G from C. argentinense. FACS analysis of yeast displayed scFv binding (A) BoNT/Gi or (B) native BoNT/G. Yeast displayed scFv were stained with (A) 50 nM inactive BoNT/Gi, or (B) Approximately 50 nM of BoNT/G (based on purity estimated from SDS-PAGE) with binding detected using anti-BoNT/Gi mouse serum. BoNT/Gi or BoNT/G binding mean fluorescence intensity is shown on the y-axis, and the mean scFv display fluorescence intensity level is shown on the x-axis. A humanized version of 6G6 (hu6G6) was used instead of 6G6 for this experiment.