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. 2023 May 22;11(5):1359. doi: 10.3390/microorganisms11051359

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluation of mRNA expression of genes coding for adherens junction protein, tight junction protein, and mucin in the colons of mice with DSS-induced colitis. mRNA extracted from colonic tissue was prepared for real-time PCR. mRNA levels of genes coding for (A) E-cadherin, (B) claudin3, (C) occludin, (D) ZO-1, and (E) MUC2 proteins were evaluated using each primer and normalized against GAPDH (n = 5). The significance of differences among the DSS-treated groups were assessed using the Mann–Whitney U test, with the level of significance set at p < 0.05 (*). PCR, polymerase chain reaction; ZO, zonula occludens; MUC, mucin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; DSS, dextran sulfate sodium.