Table 2.
NBD-PS Transport in Microsomal Vesicles Reconstituted in the Presence of Solubilized Membrane Proteins as Indicated
| Controls | −ATPa,b (%) | +ATPa,c (%) |
|---|---|---|
| Yeast DRS2 microsomes | 48 ± 5 | 29 ± 4 |
| Yeast drs2 microsomes | 51 ± 3 | 42 ± 6 |
| Yeast DRS2 microsomes + vanadate (200 μM) |
46 ± 4 | 45 ± 3 |
| No added protein | 49 ± 3 | 51 ± 3 |
| Plasma membrane H+-ATPase | 47 ± 3 | 46 ± 2 |
a
Values are given as inner leaflet NBD-PS (%) and are the means ±sd of three measurements from three independent reconstitution experiments. Protein-to-lipid ratios were 1:15.
b
ATP was omitted from the assay.
c
ATP (3 mM) was added to start the reaction.