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Growth of Nyctotherus velox in vitro. Effect of temperature, soluble nutrients, and β-sitosterol on the trophozoites and cyst counts. Medium A, complex cultivation medium. Medium C, cultivation medium without soluble nutrients (peptone, glucose, and vitamins). SRS, rice starch covered with β-sitosterol. RS, rice starch. Probability values: * p < 0.05, ** p < 0.01, *** p < 0.001.
