Figure 2.

Phylogeny of the Nyctotherus velox and Nyctotherus archispirostreptae n. sp., the ciliates isolated from the hindgut content of the millipede Archispirostreptus gigas. Sequences obtained in this study are in the bolded text (3E, 8E, 11E, single-cell samples from the hindgut contents; 2K–10K, single-cell samples from the culture). Phylogeny of the Nyctotherus species was based on 18S rDNA gene sequences. The phylogenetic tree was constructed using Bayesian Inference (BI). The topology is further supported by Maximum Likelihood (ML) and Maximum Parsimony (MP). The first number at the nodes, followed by ML and MP bootstrap values, indicates posterior probabilities. The star indicates the maximum possible support (posterior probability 1, bootstrap 100). The presence of the dash denotes posterior probability less than 0.90 and bootstrap less than 50. The scale bar represents two substitutions per 100 nucleotide positions. The sequences are listed in the Supplementary Materials.