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HILIC-UV profile of (a) RNase A; (b) RNase A conjugated with 20 purified by precipitation with EtOAc, conjugation performed in non-optimized conditions; (c) RNase A conjugated with 20 purified by HILIC, conjugation performed in non-optimized conditions; (d) RNase A conjugated with 20 purified by HILIC, conjugation performed in optimized conditions. Detection was performed at 214 nm. Numbers indicate the incorporated Man2 units.
