Figure 2.

RPS2 Activity of RPS2 Derivatives Measured by a Transient Expression Assay.

RPS2 activity of RPS2 derivatives in response to avrRpt2 was measured by using a transient expression assay, with a decrease in GUS activity being a measure of the RPS2 activity and with luciferase activity as a normalization factor for the transformation efficiency, as previously described (Leister et al., 1996). Similar assays were performed with RPS2 derivatives in the absence of avrRpt2. For each derivative, the data are expressed as the percentage of the normalized GUS activity observed in the presence of avrRpt2 divided by that in the absence of avrRpt2. The more active a derivative, the less its relative GUS activity. Error bars represent the standard deviation. WT, wild type.