Table 2.
Plasmid Construct
|
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---|---|---|---|
Activity | pKEx4tr | pBI221 | pKEx4tr-G |
Relative GUS activity (mean ± se) in biolistically bombarded plants | −0.2 ± 0.2a | 100 ± 6a | 286 ± 29a |
Relative GUS activity (mean ± se) in E. coli | 0.02 ± 0.01b | 100 ± 22b | 0.07 ± 0.02b |
The measured GUS activities were normalized by the corresponding luciferase activities. All values were renormalized, setting the mean value for pBI221 to 100. Four replicates were measured for each construct.
The measured GUS activities were normalized by the relative copy numbers of the plasmids. All values were renormalized, setting the mean value for pBI221 to 100. Four independent transformants were analyzed for each construct.