Fig. 2.

Aptamers as detection reagents for SARS-CoV-2 N protein and inactivated virus in a hybrid ELASA format. Aptamers 1 and 5 were evaluated as detection reagents in a hybrid ELASA format where an antibody was used for capture and aptamers were used as detection reagents. A. Detection of SARS-CoV-2 Alpha recombinant nucleocapsid protein or B. inactivated virus. C. Aptamers were evaluated for their ability to detect N protein from SARS-CoV-2 viral strains of interest (200 ng). D. Detection of SARS-CoV-2 Omicron recombinant nucleocapsid protein or E. inactivated virus. The error bars indicate standard deviation and statistical significance is indicated when p < 0.05 and was calculated using one-way ANOVA with Šidák multiple comparisons test (Figures A, B, D and E) and Welches T Test (Figure C).