. 2023 May 10;28(10):4011. doi: 10.3390/molecules28104011

Table 3.

Polyphenol content and polyphenol profile identified by UPLC-PDA-MS-MS.

					CB	BD	BA	BL	BW
Total polyphenols content (mg GAE/L)					134.0 ^a ± 0.2	180.3 ^b ± 0.3	181.2 ^b ± 0.4	276.3 ^d ± 0.3	252.3 ^c ± 0.7
Compound (mg/L)	Rt (min)	λ_max (nm)	(M-H) m/z
Compound (mg/L)	Rt (min)	λ_max (nm)	MS	MS/MS
Neo-chlorogenic acid	2.15	288 sh, 324	353	191	<LOQ	0.82 ^c ± 0.05	0.68 ^b ± 0.01	0.48 ^a ± 0.02	0.67 ^b ± 0.03
Chlorogenic acid	2.70	299 sh, 327	353	191	<LOQ	5.86 ^c ± 0.03	7.30 ^d ± 0.01	1.20 ^a ± 0.02	3.81 ^b ± 0.02
Unspecified caffeic acid derivative	3.02	298 sh, 322	507	353, 161	<LOQ	0.25 ^b ± 0.08	0.26 ^b ± 0.09	0.17 ^a ± 0.02	0.53 ^c ± 0.08
K-3-O-p-hydroxybenzoyl-glc	3.56	264, 347	567	447, 285	0.65 ^d ± 0.01	0.44 ^b ± 0.09	0.32 ^a ± 0.01	0.30 ^a ± 0.01	0.49 ^c ± 0.02
Caffeoylglucaric acid	3.61	299 sh, 327	371	179	0.41 ^c ± 0.01	0.38 ^b ± 0.00	0.36 ^b ± 0.00	0.29 ^a ± 0.00	0.59 ^d ± 0.02
Dihydroquercetin 3-O-glc	3.73	255, 354	465	303	0.98 ^d ± 0.02	0.80 ^c ± 0.02	0.42 ^a ± 0.02	0.41 ^a ± 0.02	0.70 ^b ± 0.03
Q-3-O-glc-pent	3.88	253, 354	595	301	0.17 ^a ± 0.00	0.86 ^d ± 0.04	0.74 ^c ± 0.00	0.71 ^c ± 0.00	0.53 ^b ± 0.02
Q-3-O-rut	4.16	255, 355	609	301	0.06 ^a ± 0.00	0.92 ^c ± 0.02	2.39 ^d ± 0.07	<LOQ	0.73 ^b ± 0.00
Q-3-O-glc	4.35	253, 352	463	301	0.28 ^c ± 0.00	0.86 ^d ± 0.03	0.15 ^a ± 0.01	<LOQ	0.21 ^b ± 0.00
Total					2.54 ^a ± 0.05	11.19 ^d ± 0.32	12,70 ^e ± 0.17	3.05 ^b ± 0.46	8.27 ^c ± 0.22

Data are expressed as a mean values (n = 3) ± SD; SD—standard deviation. Mean values within a row with different letters are significantly different (p < 0.05). CB—wheat beer without added fruit; BD—wheat beer with added kamchatka berry “Duet”; BA—wheat beer with added kamchatka berry “Aurora”; BL—wheat beer with added haskap “Lori”; BW—wheat beer with added haskap “Willa”. <LOQ—below limit of quantification; K—kaempferol; Q—quercetin; glc—glucoside; rut—rutinoside; pent—pentoside.