Figure 3.

Prediction and testing of metabolites correlating with TEER increase. (A) Most important bacterial metabolites for the prediction of normalized TEER after 8 h by random forest regression. %IncMSE represents the relative impact of a metabolite on the accuracy of the model. Model performance shown inside the RF plot. The red line is fitted by linear regression. (B–E) Transepithelial electrical resistance (TEER) readings of Caco-2 monolayers challenged with single metabolites for 8 h with DMEM AB-free used as control. Error bars indicate the SD of n = 3. HA, hydroxysiocaproic acid; EPA, Eicosapentanoeic acid; ILA, Indole-3-lactic acid.