Figure 4.

Glucagon-like peptide-1 (GLP-1) secretion from STC-1 cells after bacterial co-incubation. Ranking of 42 bacterial strains based on ability to elicit secretion of GLP-1 from STC-1 monolayers. GLP-1 total was measured after 3 h incubation via the MSD platform. Each blue bar corresponds to Bifidobacterium strains and dark gray to Lactobacillus strains. Control is untreated cells in HEPES buffer. The black dotted line indicates baseline of control GLP-1 secretion = 1. Data shown as mean of triplicates normalized to baseline and error bars are SD. Results were compared with unstimulated control via a one-way ANOVA with Dunnett’s post-hoc analysis for multiple comparisons: **** p < 0.0001, *** p < 0.001, * p < 0.05 to control test.