Figure 6.

Indole metabolites inhibits de novo lipogenesis in primary hepatocytes. Results of de novo lipogenesis upon 3 h stimulation with high-quantity microbial-derived indole metabolites; indole-3-carbaldehyde (ICA) (A), indole-3-carbaldehyde (ILA) (B), or indoleacetaldehyde (IAA) (C). Allosteric AMPK activator (MK-8722: MK) was used as the positive control. Results are depicted as percentage de novo lipogenesis where media control (vehicle) is set to 100%. Data shown as mean of triplicates (n = 3) and error bars are SD. One-way ANOVA followed by Dunnett’s post-hoc analysis for multiple comparisons was performed. **** p < 0.0001, ** p < 0.01, vs. control.