Table 5.

Antibacterial activity and MIC of the various extracts of Caulerpa racemosa against tested microorganisms.

		Zone of Inhibition (mm)
Extract	Bacterial Strain	Control (Streptomycin)	50µg/mL	100 µg/mL	150 µg/mL	200 µg/mL	MIC µg/mL
Methanol	Aeromonas hydrophila	25.5 ± 2.12	-	15.5 ± 0.72 **	17.5 ± 2.12 **	21.5 ± 2.12 *	100
	Aeromonas veronii	29 ± 1.41	-	20 ± 2.82 *	24 ± 2.83 *	27 ± 0.71 *	100
	Aeromonas salmonicida	26.5 ± 0.70	-	16 ± 1.41 **	17.5 ± 2.12 **	23.5 ± 0.71 *	100
	Pseudomonas aeruginosa	29 ± 1.41	-	11.5 ± 2.12 **	14 ± 0.70 **	19.5 ± 2.12 *	200
	Staphylococcus aureus	26.5 ± 0.70	-	12.25 ± 1.06 **	15 ± 1.41 **	17.5 ± 0.71 **	200
	Klebsiella pneumoniae	26 ± 2.83	-	12 ± 1.41 **	14 ± 1.41 **	17.75 ± 1.06 **	200
Ethanol	Aeromonas hydrophila	30 ± 2.83	-	12.5 ± 0.71 **	16 ± 1.41 **	19.5 ± 0.71 *	100
	Aeromonas veronii	29.5 ± 2.12	-	16.5 ± 2.12 **	19 ± 2.83 *	25 ± 0.35 *	100
	Aeromonas salmonicida	32.75 ± 0.35	-	14.5 ± 2.12 **	17.5 ± 0.71 **	21.25 ± 0.35 *	100
	Pseudomonas aeruginosa	24.5 ± 0.71	-	12 ± 1.41 **	14 ± 1.41 **	16.5 ± 0.71 **	200
	Staphylococcus aureus	24.5 ± 0.71	-	10.75 ± 0.35 **	11.5 ± 0.71 **	13.5 ± 0.71 **	200
	Klebsiella pneumoniae	29.5 ± 0.71	-	-	11.25 ± 1.06 **	13.5 ± 2.12 **	200
Acetone	Aeromonas hydrophila	28.5 ± 2.12	-	11 ± 1.14 **	13.5 ± 0.71 **	16 ± 1.41 **	100
	Aeromonas veronii	31 ± 1.41	-	14.5 ± 2.12 **	18 ± 2.83 **	21.5 ± 0.71 *	100
	Aeromonas salmonicida	27.5 ± 2.12	-	15.5 ± 0.71 **	16 ± 1.41 **	18 ± 1.41 **	100
	Pseudomonas aeruginosa	29 ± 1.41	-	-	10.5 ± 0.71 **	11.5 ± 0.71 **	200
	Staphylococcus aureus	25.5 ± 2.12	-	10.5 ± 0.71 **	11.5 ± 0.71 **	11.75 ± 1.06 **	200
	Klebsiella pneumoniae	28.5 ± 0.71	-	-	10.5 ± 0.71 **	13.5 ± 0.71 **	200
Ethyl acetate	Aeromonas hydrophila	29 ± 0.71	-	11 ± 1.41 **	12 ± 2.82 **	15.5 ± 0.71 **	200
	Aeromonas veronii	30.5 ± 0.71	-	12 ± 2.83 **	15.5 ± 2.12 **	20 ± 1.41 *	100
	Aeromonas salmonicida	29.5 ± 2.12	-	12.5 ± 0.71 **	13.5 ± 2.12 **	15.5 ± 0.71 **	100
	Pseudomonas aeruginosa	29 ± 1.14	-	-	-	10.5 ± 0.71 **	200
	Staphylococcus aureus	27.25 ± 0.35	-	11 ± 0.35 **	11 ± 0.35 **	11.5 ± 0.71 **	200
	Klebsiella pneumoniae	26 ± 2.83	-	-	11.5 ± 0.71 **	11 ± 1.41 **	400
Petroleum ether	Aeromonas hydrophila	26.5 ± 0.71	-	-	-	12 ± 1.41 **	400
	Aeromonas veronii	28.5 ± 0.71	-	11.5 ± 2.12 **	11.5 ± 0.71 **	14.25 ± 1.06 **	200
	Aeromonas salmonicida	26.5 ± 0.71	-	11.5 ± 0.71 **	10.75 ± 1.06 **	11.5 ± 0.71 **	200
	Pseudomonas aeruginosa	29.5 ± 2.12	-	-	11 ± 0.71 **	12 ± 1.41 **	400
	Staphylococcus aureus	28.5 ± 2.12	-	-	10.5 ± 0.71 **	12.5 ± 0.71 **	400
	Klebsiella pneumoniae	27 ± 1.41	-	-	-	11.5 ± 2.12 **	400
Hexane	Aeromonas hydrophila	27.5 ± 0.71	-	-	10.5 ± 0.71 **	12 ± 1.41 **	400
	Aeromonas veronii	30 ± 0.71	-	10.5 ± 0.71 **	13.5 ± 0.71 **	16 ± 1.41 **	200
	Aeromonas salmonicida	32 ± 1.41	-	10.5 ± 0.71 **	10.5 ± 0.71 **	12 ± 1.41 **	400
	Pseudomonas aeruginosa	29 ± 1.41	-	-	-	13.5 ± 0.70 **	400
	Staphylococcus aureus	26 ± 1.41	-	-	11 ± 1.14 **	12 ± 2.82 **	400
	Klebsiella pneumoniae	27.5 ± 0.71	-	-	-	12.5 ± 2.12 **	200

Each result represents the mean±standard deviation (n = 3), and asterisks indicate significant differences between the control and different concentrations of Caulerpa racemosa extracts. “-” indicates no activity.