Table 2.
Distribution of tumour markers
| Scoring group | Totally negative | Focal, weak | Focal, moderate/strong | Diffuse, weak | Diffuse, moderate/strong | No viable tumour cells |
|---|---|---|---|---|---|---|
|
D4D6 TMA N (%) |
837 (90.9) | 26 (2.8) | 1 (0.1) | 19 (2.1) | 8 (0.9) | 30 (3.3) |
| Mean/median H-score (range) | 0 | 18.7/20 (5–40) | 35/35 (35) | 82.6/80 (60–130) | 221.3/220 (160–300) | |
| FISH positiv | 2/68 | 0/5 | 0/1 | 0/19 | 3/7 | 0/1 |
| NGS | 69/837 | 7/26 | 1/1 | 18/19 | 8/8 | 1/30 |
| –RNA/ Fusions |
ALK: 1 FGFR3: 1 MYB: 1 RET: 1 Failed: 2 Negative: 63 |
Negative: 6 Failed: 1 |
Negative: 1 |
Met exon 14 skipping: 1 Failed: 1 Negative: 16 |
ROS1: 3 Negative: 5 |
Negative: 1 |
|
SP384 TMA N (%) |
827 (89.8) | 35 (3.8) | 1 (0.1) | 24 (2.6) | 15 (1.6) | 19 (2.1) |
| Mean/median H-score (range) | 0 | 25.3/30 (5–40) | 40/40 (40) | 100.4/100 (60–140) | 204/200 (120–300) | |
| FISH positive (Positive/total tested) | 2/56 | 0/7 | 0/1 | 0/21 | 3/15 | 0/1 |
| NGS | 60/827 | 4/35 | 1/1 | 23/24 | 15/15 | 1/19 |
| –RNA/Fusions |
FGFR3: 1 MYB: 1 Failed: 2 Negative: 56 |
RET: 1 Negative: 3 |
Negative: 1 |
ALK: 1 MET: 1 Failed:1 Negative: 20 |
ROS1: 3 Failed: 1 Negative: 11 |
Negative: 1 |
Negative IHC is defined as totally negative or focal and weak staining. In this table we have separated the negative group in totally negative and focal and weak. For details about the results of the DNA NGS (copy number variation (CNV) and hotspot mutations), see Additional file 1: Table S5