Figure 1.

Phenotypic and Molecular Characteristics of Susceptible Mla1 Mutants.

(A) Primary leaf tip fragments were collected from the infected parent lines, AlgR (resistant) and AlgS (susceptible), and the Mla1 mutants at 7 days after inoculation with the K1 (AvrMla1) powdery mildew isolate. Powdery mildew hyphae are visible as white spots.

(B) to (D) Results of sequential hybridization to a single blot (HindIII digestion) with probes RGH1a (B), MWG2083 (C), and MWG2197 (D) using normal stringency wash conditions (2 × SSC, 0.5% SDS for 30 min and 0.1 × SSC, 0.5% SDS for 30 min at 65°C; 1× SSC is 0.15 M NaCl and 0.015 M sodium citrate). Arrows indicate the deletions in M508 and M510.