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DNA Hybridization Analysis of Mla1 Mutants Using Mla1 Probes.
(A) A genomic DNA gel blot of the parents and Mla1 mutant lines digested with HindIII hybridized with MLA1-NB probe.
(B) The same DNA gel blot hybridized with MLA1-LRR probe.
The arrows indicate the deletions in M508 and M510. Normal stringency wash conditions (2 × SSC, 0.5% SDS for 30 min and 0.1 × SSC, 0.5% SDS for 30 min at 65°C) were used for both probes.
