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. 2023 May 27;14:3062. doi: 10.1038/s41467-023-38919-2

Fig. 5. macroH2A2 represses enhancer elements linked to neurodevelopmental genes.

Fig. 5

a, b Examples of ATAC-seq enhancer peaks gained upon knockdown of MACROH2A2/macroH2A2. c Expression of eRNA at the COL20A1 locus shown in (b) in control versus knockdown cells. Two biological replicates were used to generate RNA-seq libraries. P value was obtained by a two-tailed unpaired T-test with Welch’s correction. d Differentially accessible chromatin regions identified upon MACROH2A2/macroH2A2 knockdown in a scATAC-seq sample from four primary glioblastoma resections. The horizontal axis represents individual cells in the specimens, with differentially accessible regions listed along the Y axis. e Accessibility at the COL20A1 enhancer in a scATAC-seq dataset generated from four primary GBM surgical specimens. Samples are separated by arcs. f Top positively enriched motifs at COL20A1 accessible cells in primary glioblastoma resections. g Top negatively enriched motifs at COL20A1 accessible cells in primary glioblastoma resections.