Table 2.

Relative primers used for PCR, real-time PCR, stem-loop sequences.

Name	Sequences
PCR analysis (3′UTR)
Top2A	Sense Anti-sense	5′ CCGCTCGAGACCAAGCCCAAGACTGGTTT 3′ 5′ ATAAGAATGCGGCCGCGCACATAAGAGGCTGAGTGT 3′
MALAT1	Sense Anti-sense	5′ CCGCTCGAGTTCAGTTGTGTGTAAGCAAGT 3′ 5′ ATAAGAATGCGGCCGCAGATAATGTTCTCATCAGTAGTAAG 3′
qPCR primer
Top2A	Sense Anti-sense	5′ CATTGAAGACGCTTCGTTATGG 3′ 5′ CAGAAGAGAGGGCCAGTTGTG 3′
MALAT1	Sense Anti-sense	5′ CTTCCCTAGGGGATTTCAGG 3′ 5′ GCCCACAGGAACAAGTCCTA 3′
miR-561	Sense Anti-sense	5′ CGCTCCAAAGTTTAAGATCCTTGAAG 3′ 5′ AGACTGCACCTGTCCGG 3′
GAPDH	Sense Anti-sense	5′ GAAAGCCTGCCGGTGACTAA 3′ 5′ GCGCCCAATACGACCAAATC 3′
U6	Sense Anti-sense	5′ CGCAAGGATGACACGCAAATTC 3′ 5′ AGACTGCACCTGTCCGG 3′
siRNA and mimic sequences
siRAN1#MALAT1		5′-GGCUUAUACUCAUGAAUCUTT-3′
siRAN2#MALAT1		5′-GGGCUUCUCUUAACAUUUATT-3′
miR-561	Sense Anti-sense	5′ CAAAGUUUAAGAUCCUUGAAGU 3′ 5′ ACUUCAAGGAUCUUAAACUUUG 3′
Negative control		UUCUCCGAACGUGUCACGUTT
miRNA inhibitor negative control		CAGUACUUUUGUGUAGUACAA
miR-561 stem-loop		GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTTCA