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. 2023 May 27;14:3059. doi: 10.1038/s41467-023-38774-1

Fig. 2. Elevated mutation rate of the DNA polymerase δ mutant line.

Fig. 2

A Mutation accumulation assay comparing Dd2-WT with three clones of Dd2-Polδ. All lines were cultured in parallel for 100 days (~50 generations). Parasites were sampled for clonal isolation every 20 days and subsequently harvested for genomic DNA extraction. Whole-genome sequencing was performed on samples collected on day 0, 20, 40, 60, 80 and 100. B The number of unique SNVs in the exome, non-coding and core genome regions of Dd2-WT and Dd2-Polδ lines were identified by subtracting from the SNVs found on day 0. Each point represents one clone, where n=the following number of independent clones (WT:12, E8:12, F11:14, H11:11). The median line is shown and 25th/75th percentile bounded by the box, with whiskers showing min-max. A two-tailed Wilcoxon matched-pairs signed-rank test showed statistically significant differences for the Dd2-Polδ clones relative to Dd2-WT, with p values as indicated (a:0.0049; b:0.0015; c:0.002; d:0.002; e:0.002; f:0.0298; g:0.0005; h:0.001). C Genomic position of SNVs, colour-code by parasite line. D The mutation rates of Dd2-WT (n = 12) and Dd2-Polδ clone E8 (n = 12), F11 (n = 14) and H11 (n = 11), data are presented as mean + /−SD (with values and 95% confidence intervals shown in Table 1). Source data are provided as a Source Data file.