Figure 4.

CIP4 Is a Competent Transcriptional Activator in Plant Cells.

(A) The constructs used in the assay. The reporter (GAL4op-LUC) and the reference (35S-GUS), which contains a translational enhancer element (TLE), were mixed together with the effectors (GAL4 DNA binding domain fusion proteins) and introduced into tobacco leaves by the microprojectile bombardment method. Numbers in parentheses indicate the amino acid residue coordinates of the proteins, arrows indicate the direction of transcription, and 35S Dual indicates the 35S promoter with two copies of the enhancer element. The GAL4_UAS indicates the GAL4 DNA binding site in the promoter.

(B) Transient activation of the reporter in plant cells. LUC reporter activity was normalized to GUS activity. Average and sd values of the normalized LUC activity (LUC/GUS) are shown. At least six independent tests were performed for each sample. In the case of GAL4–CIP4, two concentrations of the effector plasmids (×2 and ×1) were tested.