Figure 2 |. Nmur1^Cre deletes amphiregulin within ILC2s with high efficiency.

a, b, Representative flow cytometry analysis of AREG expression in WT ILC2s (a) and percentages of AREG⁺ ILC2s isolated from SI (n=13 mice), colon (n=13 mice), MLN (n=13 mice), and spleen (n=8 mice) (b). c, d, Representative flow cytometry analysis of AREG⁺ ILC2s (c) and percentages of IL-5⁺ or NMUR1⁺ cells within AREG⁺ ILC2s in MLN isolated from WT mice (n=4 mice) (d). e, f, Representative flow cytometry analysis of ILC2s (e) and percentages of AREG⁺ ILC2s in MLN isolated from Areg^fl (n=9 mice), Areg^ΔRed5 (n=9 mice) and Areg^ΔILC2 (n=7 mice) (f). g-i, Representative flow cytometry analysis of ILC2s (g), percentages of ILC2s within total ILCs (h), and absolute numbers of ILC2s (i) in the colons of Areg^fl (n=4 mice) and Areg^ΔILC2 (n=5 mice). j, k, Representative flow cytometry analysis of IL-5 and IL-13 production in ILC2s (j) and percentages of IL-5⁺IL-13⁺ DP, total IL-5⁺ and total IL-13⁺ cells within ILC2s (k) in the colons of Areg^fl (n=4 mice) and Areg^ΔILC2 (n=5 mice). Data in b are pooled from three independent experiments. Data in d, h, i, and k are representative of two independent experiments. Data from f are pooled from two independent experiments. One-way ANOVA with Tukey multiple comparisons test (b, f). Unpaired two-sided t-test (h, i, and k). Paired two-sided t-test (d). P values are presented where appropriate. ns, not significant. Data are represented as means ± S.E.M. DP – double positive.