Extended Data Figure 3 |. Comparison of efficiency of ILC2-depletion and its impact on other immune cells in R26-DTR^Nmur1 and R26-DTR^Red5 mice.

a-c, R26-DTR (n=11 mice) and R26-DTR^Nmur1 (n=7 mice) were treated with 2 daily injections of DT. The small intestine (SI), colon, mesenteric lymph nodes (MLN), and spleen were harvested for analysis after resting the mice for 2 days. Abundances of non-ILC2 innate lymphoid cell subsets (a), adaptive lymphocytes (b) or myeloid cells and granulocytes (c) within the indicated tissues. d-g, R26-DTR (n=7 mice) and R26-DTR^Red5 (n=6 mice) were treated with 2 daily injections of DT and rested for 2 days. Representative flow cytometry analysis of ILC2s in the colon, pre-gated on total ILCs (d) (Total ILCs gated as live CD45⁺Lin⁻CD90⁺CD127⁺ events. Lin: CD3ε, CD5, CD11b, CD11c, FcεRI, B220). Percentages of ILC2s out of total ILCs in the SI, colon, MLN, and spleen (e). Abundances of innate lymphoid cell subsets (f), adaptive lymphocytes (g). Data in a-c and e-g are pooled from two independent experiments. Two-way ANOVA with Šídák multiple comparisons test (a-c, e-g). P values are presented where appropriate. ns, not significant. Data are represented as means ± S.E.M.