TABLE 1.

RNA integrity preservation.

Freezing method	RIN value
	Replicate 1	Replicate 2	Replicate 3
Liquid nitrogen	9.40	9.50	9.30
Snap freezer	9.10	9.30	9.20
+2 h Control sample	9.40	9.20	9.30

Note: RNA integrity of cell line samples processed with different freezing methods. Aluminum vials with lysates of K562 suspension cell line were alternatingly snap‐frozen in the snap freezer or in liquid nitrogen. Three samples were left at room temperature for 2 h before freezing in liquid nitrogen as a positive control sample. RIN, RNA integrity numbers.