Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Total OXPHOS	Abcam	Cat#: ab110411; RRID:AB 2756818
CHK1	Abcam	Cat#: ab40866; RRID:AB 726820
MT-ND1	Elabscience	Cat#: E-AB-32173; RRID:AB 2881266
MT-CYTB	ProteinTech	Cat#: 55090–1-AP
FLAG	Sigma	Cat#: F7425; RRID:AB_439687
Anti-Puromycin	Sigma	Cat#: MABE343; RRID:AB 2566826
AKT2	Cell Signaling Tech	Cat#: 3063; RRID:AB 2225186
CHK1-S296	Cell Signaling Tech	Cat#: 2349; RRID:AB 2080323
Anti-biotin, HRP-linked	Cell Signaling Tech	Cat#: 7075; RRID:AB 10696897
YH2AX	Cell Signaling Tech	Cat#: 2577; RRID:AB 2118010
FLAG	Cell Signaling Tech	Cat#: 14793; RRID:AB 2572291
LAMIN A/C	Cell Signaling Tech	Cat#: 4777; RRID:AB 10545756
HA	Cell Signaling Tech	Cat#: 3724; RRID:AB 1549585
TOM20	Cell Signaling Tech	Cat#: 13929; RRID:AB 2631994
p-ACTIN	Cell Signaling Tech	Cat#: 4970; RRID:AB 2223172
MT-ATP6	ProteinTech	Cat#: 55313–1-AP; RRID:AB 2881305
SSBP1	ProteinTech	Cat#:12212–1-AP; RRID:AB 2195320
DNA	Progen	Cat#: 61014; RRID:AB 2750935
CHK1	Santa Cruz	Cat#: SC-8408; RRID:AB 627257
HRP-labeled anti-mouse	Santa Cruz	Cat#:SC-2005; RRID:AB 631736
HRP-labeled anti-rabbit	Santa Cruz	Cat#: SC-2030; RRID:AB 631747
Anti-mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 594 Conjugate)	Cell Signaling Tech	Cat#: 8890; RRID:AB 2714182
Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor(R) 647 Conjugate)	Cell Signaling Tech	Cat#: 4414; RRID:AB 10693544
Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor(R) 488 Conjugate)	Cell Signaling Tech	Cat#: 4412; RRID:AB 1904025
Bacterial and virus strains
DH5a	Fisher Scientific	Cat#:18265017
Chemicals, peptides, and recombinant proteins
RPMI-160	Corning	Cat#: ICN1646454
FBS	Corning	Cat#: 35–010-CV
DMEM	Corning	Cat#: 10–013-CV
L-Glutamine	Corning	Cat#: 25–005-CI
Glutamax I	Invitrogen	Cat#: 35050061
Penicillin-Streptomycin	Millipore	Cat#: P0781
Blasticidin	Fisher Scientific	Cat#: 50712728
Puromycin	Sigma-Aldrich	Cat#: P8833
Streptavidin agarose resin	Fisher Scientific	Cat#: 20347
Sequencing grade modified trypsin	Promega	Cat#: V5111
2-Iodoacetamide	Fisher Scientific g	Cat#: ICN10035125
Iodoacetamide-desthiobiotin	Santa Cruz	Cat#: sc-300424
Nitroso-DTB	This Study	N/A
DMSO	Fisher Scientific	Cat#: MT- 25950CQC
Urea	VWR Intl	Cat#: 97063–798
CHAPS hydrate	Sigma-Aldrich	Cat#: C3023
DTT	Fisher Scientific	Cat#: BP1725
Micro bio-spin column	Bio-rad	Cat#: 7326204
Protease Inhibitor Cocktail	Sigma-Aldrich	Cat#: 5892791001
PhosSTOP™	Sigma-Aldrich	Cat#: 4906845001
Polybrene	Santa Cruz	Cat#: 134220
X-tremegene 9 transfection reagent	Sigma-Aldrich	Cat#: 6365787001
X-tremegene HP transfection reagent	Sigma-Aldrich	Cat#: 6366236001
Anti-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat#: A2220
Anti-HA Magnetic Beads	Fisher Scientific	Cat#: 88836
Cell Titer Glo™ reagent	Promega	Cat#: G7570
Prolong Gold Antifade Mount	Life Technologies	Cat#: P36930
Formaldehyde	Sigma	Cat#: UN3334
Arsenic Trioxide	Fisher Scientific	Cat#: R0817000500
β-Lapachone	Cayman Chemical	Cat#: 15021
Doxorubicin	Cayman Chemical	Cat#: 15007
Elesclomol	Selleckchem	Cat#: S1052
Cisplatin	Cayman Chemical	Cat#: 13119
Bleomycin	Cayman Chemical	Cat#: 13877
5-Fluorouracil	Cayman Chemical	Cat#: 14416
Sulfasalazine	Cayman Chemical	Cat#: 15025
Auranofin	Cayman Chemical	Cat#: 15316
2-Methoxyestradiol	Cayman Chemical	Cat#: 13021
NOV-002	MedKoo	Cat#: 202030
Hydrogen peroxide solution	Sigma	Cat#: 216763
Doxycycline	Sigma	Cat#: D3447
MitoTEMPO	Sigma	Cat#: SML0737
N-acetyl-L-Cysteine	Sigma	Cat#: 20261
Carboxy-DCFDA (5-(and-6)-Carboxy-2’,7’- Dichlorofluorescein Diacetate)	Fisher Scientific	Cat#: C369
MitoSOX™ Red	Fisher Scientific	Cat#: M36008
L-ALA	TCI America	Cat#: A0179
D-ALA	TCI America	Cat#: A0177
L-Buthionine-sulfoximine	Sigma	Cat#: B2515
Trolox	Cayman	Cat#: 10011659
Glutathione reduced ethyl ester	Sigma	Cat#: G1404
L -(+)-Ergothioneine	Sigma	Cat#: 497-30-3
S1QEL1.1	Sigma	Cat#: SML1948
S3QEL-2	Sigma	Cat#: SML1554
ATP, [Y-32P]	Perkin Elmer	Cat#: BLU002Z250UC
Critical commercial assays
NADP/NADPH-Glo™ Assay kit	Promega	Cat#: G9081
NAD/NADH-Glo™ Assay kit	Promega	Cat#: G9071
GSH/GSSG-Glo™ Assay	Promega	Cat#: V6611
Deposited data
Proteomics data	This study	PRIDE: PXD041138
Experimental models: Cell lines
K562	ATCC	CCL-243
HEK293T	ATCC	CRL-11268
K562-dCas9-KRAB	Gilbert et al., 2014	N/A
OVCAR3	ATCC	HTB-161
Kuramochi	JCRB	JCRB0098
OVCAR8	ATCC	CMT-929
PEO1	ECACC	10032308
CAOV3	ATCC	HTB-75
OVISE	JCRB	JCRB1043
OV90	ATCC	CRL-11732
OVCAR4	NCI/DTP	N/A
OAW28	ECACC	85101601
Recombinant DNA
Plenti: Hyper7-NLS	This study	N/A
Plenti: Mito-Hyper7	This study	N/A
Plenti: roGFP2-Orp1-NLS	This study	N/A
Plenti: Mito-roGFP2-Orp1	This study	N/A
Plenti: FLAG-DAAO-NLS	This study	N/A
Plenti: FLAG-MITO-DAAO	This study	N/A
Plenti: FLAG-Mito-DAAO-CHK1	This study	N/A
Plenti: CHK1	This study	N/A
pLJM1: FLAG-METAP2	This study	N/A
pLJM1: FLAG-CHK1	This study	N/A
pLJM1: FLAG-CHK1 (C408D)	This study	N/A
pLJM1: FLAG-CHK1 (C408S)	This study	N/A
pRK5: FLAG-METAP2	This study	N/A
pRK5: FLAG-CHK1	This study	N/A
pRK5: FLAG-CHK1(C408D)	This study	N/A
pRK5: FLAG-CHK1-Kinase domain	This study	N/A
pRK5: HA-CHK1-KA1	This study	N/A
pRK5: HA-CHK1-KA1 (C408D)	This study	N/A
Lenti-CRISPRv2: sgSSBP1–1	This study	N/A
Lenti-CRISPRv2: sgSSBP1–2	This study	N/A
Lenti-CRISPRv2: sgCTRL	This study	N/A
Pu6-BFP: sgCONTROL	This study	N/A
pU6-BFP: sgSSBP1–1	This study	N/A
pU6-BFP: sgSSBP1–2	This study	N/A
pU6-BFP: sgCHK1–1	This study	N/A
pU6-BFP: sgCHK1–2	This study	N/A
pLKO: shCTRL	This study	N/A
pLKO: shSSBP1_1	This study	N/A
pLKO: shSSBP1_2	This study	N/A
Plenti: SSBP1-HA	This study	N/A
Plenti: NLS-SSBP1-HA	This study f ^	N/A
Plenti: SSBP1-FLAG	This study	N/A
Plenti: SSBP1-shRNA mutant	This study	N/A
Plenti: SSBP1 (S67A)-HA	This study	N/A
Plenti: SSBP1 (S67D)-HA	This study	N/A
Plenti: SSBP1-gRNA mutant	This study	N/A
Plenti: SSBP1 (S67A)-gRNA mutant	This study	N/A
Plenti: SSBP1 (S67D)-gRNA mutant	This study	N/A
Software and algorithms
Prism (v7.0)	GraphPad	https://www.graphpad.com/scientific-software/prism/
FlowJo (v10.0.7)	Treestar Inc.	https://www.flowjo.com/
ZEN 2.6	Zeiss	https://www.zeiss.com
Floview 3.1	Olympus	https://www.olympus-lifescience.com/
ImageJ	NIH	https://imagej.net/software/fiji/