Figure 5.

Iron accumulates in the cell in the absence of Grx5. (A) W303–1A (wt) and MML100 (Δgrx5) cells were grown exponentially in YPD medium at 30°C to determine iron concentration in the cell and also aconitase and citrate synthase activities in total cell extracts. (B) MML313 cells growing exponentially in YPG medium at 30°C were added (time 0) with doxycycline (2 μg/ml), and total cell iron concentration was determined at different intervals after antibiotic addition. (C) Distribution of iron (relative to total protein in the fraction) between postmitochondrial fraction (PMF) and mitochondria (Mito), in MML313 cells untreated (−doxy) or treated for 24 h with doxycycline at 2 μg/ml (+doxy).