Figure 6.

Activity of Fe/S enzymes is inhibited in grx5 cells independently of intracellular iron concentration. (A) CML235 (wt) or MML19 (Δgrx5) cells were grown exponentially in YPD medium (also in the presence of 80 μM BPS in the case of MML19 cells), and total cellular iron concentration and aconitase and malate dehydrogenase activity were determined. (B) The same parameters as in A were determined in exponential YPD cultures at 30°C of W303–1A (wt), MML100 (Δgrx5), MML348 (Δaft1), and MML345 (Δgrx5 Δaft1) cells.