Figure 6.

Ad-swH3-Epi provides protection against challenge with a divergent IAV-S. Five days after challenge lungs and tracheas were removed for histopathological analysis. H&E staining of representative trachea samples are shown (A) and representative bronchioles from lungs are shown in (B). Immunohistochemistry of bronchioles against the conserved NP viral protein (C). Tracheas shown in (A) were scored for tracheitis by a board-certified pathologist blinded to the treatment groups (D). IHC distribution was recorded; higher scores correlate with deeper pulmonary infection (E). Levels of infectious virus present in the bronchioalveolar lavage was enumerated by tissue culture infectious dose (TCID₅₀) (F) and recall T cell responses were analyzed by IFN-γ ELISPOT (G). Scale bars in (A–C) are 30μM, 120μM, and 60μM, respectively. Data are presented as the mean ± SEM (n=5; one-way ANOVA with Tukey’s multiple comparison; *p < 0.05 and **p < 0.01). ns, not significant.