Table 1.
The viral features of 47 clinical isolates and the inhibitory activity of LP-19.
| HIV isolate | Viral features | Inhibitory activity of LP-19 | ||||
|---|---|---|---|---|---|---|
| P24(pg/mL) | Virus subtypes | Tropism a | TCID50/mL b | LP-19 IC50(nM) c | LP-19 IC90(nM) c | |
| 2010096 | 3.48 | B | CCR5 | 30,000 | 0.13 ± 0.01 | 1.22 ± 0.05 |
| 2010104 | 2.68 | B | CCR5/CXCR4 | 50,000 | 0.77 ± 0.02 | 5.69 ± 1.36 |
| 2010259 | 8.21 | B | CCR5 | 15,000 | 0.03 ± 0.00 | 0.26 ± 0.00 |
| 2010968 | 6.78 | B | CCR5 | 10,000 | 1.27 ± 0.01 | 3.23 ± 0.18 |
| BJ2015EU15 | 7.88 | B | CCR5 | 15,000 | 2.31 ± 0.05 | 5.92 ± 1.00 |
| GX2016EU18 | 0.25 | B | CCR5 | 15,000 | 0.08 ± 0.00 | 0.23 ± 0.01 |
| Mean for B’ | 4.88 | B | N/A | 22,500 | 0.76 ± 0.90 | 2.76 ± 2.60 |
| GX2016EU03 | 5.38 | 01_AE | CCR5 | 50,000 | 0.96 ± 0.03 | 3.71 ± 0.38 |
| GX2016EU04 | 4.41 | 01_AE | CCR5 | 50,000 | 0.74 ± 0.02 | 2.39 ± 0.12 |
| GX2016EU07 | 4.40 | 01_AE | CCR5/CXCR4 | 50,000 | 0.44 ± 0.00 | 1.50 ± 0.05 |
| GX2016EU11 | 1.53 | 01_AE | CCR5 | 30,000 | 0.88 ± 0.01 | 2.56 ± 0.18 |
| GX2016EU14 | 4.19 | 01_AE | CCR5 | 10,000 | 0.01 ± 0.00 | 0.08 ± 0.00 |
| GX2016EU17 | 7.16 | 01_AE | CCR5/CXCR4 | 30,000 | 0.02 ± 0.00 | 0.06 ± 0.01 |
| XC2014EU18 | 9.89 | 01_AE | CCR5 | 30,000 | 0.06 ± 0.00 | 0.71 ± 0.01 |
| BJ2015EU01 | 11.39 | 01_AE | CCR5 | 6,250 | 0.27 ± 0.01 | 1.03 ± 0.05 |
| BJ2015EU03 | 5.59 | 01_AE | CCR5 | 30,000 | 0.03 ± 0.00 | 0.22 ± 0.01 |
| BJ2015EU06 | 7.94 | 01_AE | CCR5/CXCR4 | 30,000 | 0.20 ± 0.04 | 0.92 ± 0.04 |
| BJ2015EU09 | 7.63 | 01_AE | CCR5 | 70,000 | 0.61 ± 0.01 | 3.18 ± 0.22 |
| BJ2015EU11 | 8.34 | 01_AE | CCR5 | 10,000 | 0.02 ± 0.00 | 0.17 ± 0.01 |
| BJ2015EU12 | 7.12 | 01_AE | CCR5 | 30,000 | 0.10 ± 0.00 | 0.97 ± 0.06 |
| BJ2015EU14* | 6.35 | 01_AE | CCR5 | 50,000 | 0.01 ± 0.00 | 0.03 ± 0.00 |
| BJ2015EU17 | 12.40 | 01_AE | CCR5 | 15,000 | 0.01 ± 0.00 | 0.05 ± 0.01 |
| Mean for 01AE | 6.91 | 01_AE | N/A | 32,750 | 0.29 ± 0.35 | 1.17 ± 1.23 |
| BJ2015EU02 | 5.47 | 07_BC | CCR5 | 30,000 | 0.34 ± 0.02 | 1.83 ± 0.13 |
| BJ2015EU04 | 5.18 | 07_BC | CCR5 | 50,000 | 0.08 ± 0.00 | 0.36 ± 0.02 |
| BJ2015EU08 | 4.52 | 07_BC | CCR5 | 70,000 | 0.51 ± 0.02 | 3.62 ± 0.23 |
| BJ2015EU13 | 4.30 | 07_BC | CCR5 | 30,000 | 0.01 ± 0.00 | 0.05 ± 0.00 |
| GX2016EU01 | 1.45 | 07_BC | CCR5 | 15,000 | 0.15 ± 0.01 | 0.99 ± 0.05 |
| GX2016EU05 | 0.32 | 07_BC | CCR5 | 15,000 | 0.33 ± 0.02 | 1.53 ± 0.09 |
| GX2016EU08* | 4.95 | 07_BC | CCR5 | 50,000 | 0.25 ± 0.02 | 1.77 ± 0.12 |
| GX2016EU12 | 5.24 | 07_BC | CCR5 | 15,000 | 0.88 ± 0.02 | 3.58 ± 0.35 |
| XC2014EU05 | 7.28 | 07_BC | CCR5 | 90,000 | 0.17 ± 0.01 | 0.91 ± 0.12 |
| XC2014EU06 | 2.88 | 07_BC | CCR5 | 3,000 | 0.72 ± 0.12 | 2.40 ± 0.59 |
| XC2014EU08 | 5.21 | 07_BC | CCR5 | 70,000 | 0.99 ± 0.02 | 2.56 ± 0.42 |
| XC2014EU10 | 1.86 | 07_BC | CCR5 | 30,000 | 0.71 ± 0.00 | 2.71 ± 0.21 |
| XC2014EU13 | 11.09 | 07_BC | CCR5 | 70,000 | 0.16 ± 0.01 | 1.23 ± 0.07 |
| XC2014EU19 | 8.99 | 07_BC | CCR5 | 70,000 | 0.02 ± 0.00 | 0.26 ± 0.00 |
| Mean for 07BC | 4.91 | 07_BC | N/A | 43,429 | 0.38 ± 0.33 | 1.70 ± 1.16 |
| GX2016EU02* | 4.46 | 08_BC | CCR5 | 50,000 | 0.78 ± 0.02 | 1.52 ± 0.20 |
| GX2016EU22 | 7.26 | 08_BC | CCR5 | 30,000 | 0.10 ± 0.00 | 1.05 ± 0.10 |
| Mean for 08BC | 5.86 | 08_BC | N/A | 40,000 | 0.85 ± 1.35 | 2.76 ± 4.28 |
| GX2016EU15 | 0.24 | URF | CCR5 | 30,000 | 0.02 ± 0.00 | 0.17 ± 0.00 |
| GX2016EU09 | 8.28 | URF | CCR5 | 10,000 | 0.15 ± 0.00 | 1.09 ± 0.04 |
| GX2016EU10 | 4.74 | URF | CCR5 | 50,000 | 0.16 ± 0.00 | 0.79 ± 0.02 |
| XC2014EU09* | 9.59 | URF | CCR5 | 30,000 | 4.36 ± 0.04 | 14.63 ± 1.04 |
| BJ2015EU16* | 6.30 | URF | CCR5 | 15,000 | 0.28 ± 0.01 | 2.62 ± 0.28 |
| BJ2015EU19 | 5.52 | URF | CCR5 | 70,000 | 0.26 ± 0.02 | 1.01 ± 0.04 |
| GX2016EU13 | 0.30 | URF | CCR5 | 30,000 | 0.36 ± 0.01 | 1.37 ± 0.11 |
| GX2016EU23 | 0.15 | URF | CCR5 | 50,000 | 0.77 ± 0.07 | 1.39 ± 0.28 |
| XC2014EU01 | 4.32 | URF | CCR5 | 70,000 | 1.87 ± 0.02 | 3.52 ± 0.28 |
| XC2014EU20 | 5.14 | URF | CCR5 | 30,000 | 0.22 ± 0.03 | 1.04 ± 0.11 |
| Mean for URF | 4.46 | URF | N/A | 38,500 | 0.44 ± 0.49 | 1.29 ± 0.33 |
| Mean | 5.49 | N/A | N/A | 36,154 | 0.50(0.01~4.36) | 1.88(0.03~14.63) |
N/A= not applicable. aHIV isolates uses co-receptor CXCR4(CXC chemokine receptor 4), CCR5 (chemokine receptor 5), or both for cells infection. bTCID50/mL (50% cells culture infectious dose) is viral titration unit. cIC50 or IC90 was 50% or 90% inhibitory concentration. The experiment was performed in triplicate, and the data presented are mean values ± standard deviations from an independent experiment. *Viruses were from National Standard Strains of Pathogenic Microorganization (WS/T812-2022) (www.nprc.org.cn).