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Metabolites in peripheral and portal serum significantly changed early after TIPS. Both ESI- (A) and ESI+ (B) models of orthogonal partial least squares discriminant analysis (OPLS-DA) of portal metabolites were separated. Both ESI- (D) and ESI+ (E) models of peripheral metabolites were separated. Volcano plots showed significant metabolic changes in portal (C) and peripheral (F) with p-value <1, variable importance in the projection (VIP) > 1, and fold change ≥2 or ≤0.5.
