Fig 5.

Select engineering applications of encapsulins. (A) Cargo encapsulation within encapsulin protein shells can have a variety of beneficial effects, including increased cargo stability over time, increased thermal stability, and increased resistance against proteases. (B) Encapsulation of a ruthenium-based metal organocatalyst based on a covalently modifiable HaloTag yielding a system able to catalyze de-N-allylation of the shown pro-fluorophore both in vitro and in vivo. Adapted with changes with open access permission from reference⁵³ via a creative common license (https://creativecommons.org/licenses/by/4.0/). (C) In vivo encapsulation of a light-controllable minimal singlet oxygen generator (mSOG) able to generate large amounts of toxic ROS via singlet oxygen species inside mammalian cancer cells resulting in cell death. Adapted with permission from reference⁵⁸. Copyright 2021 American Chemical Society (ACS). (D) In vitro assembly of gold nanoparticles encapsulated within an encapsulin protein shell using a synthetically modified TP. Adapted from reference⁵² with permission from the Royal Society of Chemistry. Copyright 2018 The Royal Society of Chemistry.