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Spg1p SPB localization requires Cdc11p function. (A) The cdc11-119 spg1-GFP (KGY3395), cdc11-123 spg1-GFP (KGY3197), and cdc11-136 spg1-GFP (KGY3561) strains were grown at 25°C and stained with Hoechst. Single and merged images are shown. “Extra” Spg1p-containing dots are indicated by arrows. (B) KGY3395, KGY3197, KGY3561, and the spg1-GFP strain (KGY2627) were grown at 25°C, then shifted to 36°C for 4 h, and stained with Hoechst.
