Figure 1.

Schematic diagram of the S. cerevisiae Fab1 and Vac7 proteins. Four domains identified within Fab1 are indicated by the hatched boxes and their amino acid positions are noted. The PCR-mutagenized region of FAB1 is denoted by the gray line and restriction sites used in gapped plasmid repair are also indicated. Vac7 contains a transmembrane domain (TMD) at its carboxy terminus (amino acids 919–941).