Figure 4.

Deletion of FIG4 suppresses the mutant phenotypes of vac7Δ mutants. (A) Vacuole morphology of the indicated strains was visualized by labeling with FM 4-64 (left) or Nomarski optics (right). (B) Quantification of radiolabeled PtdIns derivatives from wild-type, vac7Δ, and vac7Δ fig4Δ mutant strains. The normalized average of deacylated PtdIns derivatives from the indicated strains were quantified as described in MATERIALS AND METHODS. The values of each bar represents the average of three or more independent labeling experiments with SD <10%.