Figure 5.

OsWRKY31 is required for OsMKK10-2-activated immune response. The transgenic and control plants were hydroponically cultured for 10 d, treated with 10 µM Dex, and sampled at designated time points for RNA and chemical isolations. Transcript level of each gene A to H) was determined by RT-qPCR using OsUBQ as the internal reference. Values represent means ± SD of three indepent treatments, each including three seedling leaves. Values marked with different letters indicate statistically significant differences as analyzed by the SPSS software (Duncan's multiple range test, α = 0.05). I to N) Contents of compounds were determined by liquid chromatography–tandem mass spectrometry using D₆ABA as the internal standard. I to M) sampled at 6 h after the Dex treatment. N) Leaf samples at 6 and 24 h after the Dex treatment; ND, no detection; root × 10, indicating 10 times upscale the value in root. Data are means ± SD of three or four biological repeats. Significance test was evaluated using the Student's t-test by comparing with DMSO treatment or each other as bracketed (*, P < 0.05; **, P < 0.01; ***, P < 0.001).