Figure 5.

Predicted precursor RNA structure for cluster 51304+. Sequences of AGO1-associated >26-nt sRNAs were aligned to the corresponding genomic sequence and their frequency in the libraries is shown as raw counts. The secondary structure of the matching genomic sequence was predicted with RNAfold (Lorenz et al. 2011) and depicted at the bottom. The most abundant >26-nt sRNAs present in the 5′ arm or the 3′ arm of the predicted hairpin are indicated in red and blue, respectively. Fairly precise putative cleavage sites on the left side of the depicted hairpin are indicated by red and blue arrows. More imprecise putative cleavage sites in the terminal loop of the depicted hairpin are indicated by black arrowheads.