Figure 4.

PpRAP2.4 positively regulates anthocyanin biosynthesis in “Hongzaosu” pear fruit. A) Transient overexpression of PpRAP2.4 in fruit. PpARAP2.4 was cloned into pCAMBIA1301 vector with a 35S promoter, and the empty vector (pCAMBIA1301) was used as the negative control. The plasmids were transformed into A. tumefaciens EHA105. After injection, the pear fruits were placed in dark for 3 d and then treated with strong light for 3 d. Then the phenotypes were examined. B) Total anthocyanin content in fruit transiently overexpressing PpRAP2.4. C) Expression patterns of genes related to anthocyanin biosynthesis in fruit transiently overexpressing PpRAP2.4. D) Transient silencing of PpRAP2.4 in fruit. The empty vector (pTRV1 + pTRV2) was used as the negative control. Pear fruits were placed in darkness for 1 d and then treated with strong light for 6 d. E) Total anthocyanin content in fruit in which PpRAP2.4 was transiently silenced. F) Expression patterns of genes related to anthocyanin biosynthesis in fruit in which PpRAP2.4 was transiently silenced. Error bars represent the standard deviation of 3 biological replicates. Asterisks indicate significantly different values (*P < 0.05 and **P < 0.01).