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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Biochim Biophys Acta Gene Regul Mech. 2023 Mar 24;1866(2):194929. doi: 10.1016/j.bbagrm.2023.194929

Fig. 4.

Fig. 4.

SAGA’s HAT module regulates SAGA DUB activity independent of enzymatic activity. A: Quantitation of western blots of SAGA DUB activity on recombinant H2B-Ub nucleosomes (400 nM) under conditions of limiting SAGA enzyme (25 nM) with and without the SWIRM domain. B: Quantitation of western blots of SAGA DUB activity on HeLa nucleosomes with ~50 nM H2B ubiquitinated nucleosome. SAGA DUB activity is compared with and without the SWIRM domain, with excess SAGA, 100 nM. C: WT SAGA DUB (25 nM) activity on recombinant H2B-Ub nucleosomes (400 nM) in absence and presence of acetyl CoA (10 μM). D: Quantitation of C and replicate.