Table 2.2.
Yeast strain name | Background strain | Insertions and deletions |
---|---|---|
YCW14 | BY4741 | Spt7-TAP::His3MX6 |
YCW20 | BY4741 | Spt7-TAP::His3MX6, Ada2 Δ residues 349–434 substituted with KanMX |
YHY81 | BY4705 | Sgf73-3HA::His3MX6, Δada2::KanMX, Ada2 with N terminal 3× FLAG tag |
YHY83 | BY4705 | SPT7-(Flag1)-TAP::TRP1, Δada2::KanMX, Ada2 ORF Δ residues 353–434 with 3xFlag on N-terminus, 491 bp promoter sequence, 100 bp of sequence past stop codon |