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Effects of Ca-buffer injection on the first and second cleavages
| wave1a | wave2a | 1st cleavageb | 2nd cleavageb | |
|---|---|---|---|---|
| 45 mM KCl | 7 /9 | 9 /9 | 9 /9 | 9 /9 |
| dibromoBAPTA, 0.9 mM | 2 /13c | 4 /13c | 13 /13 | 1 /13 |
| EGTA, 0.9 mM | 0 /9 | 1 /9 | 9 /9 | 9 /9 |
Number of the eggs in which Ca wave was observed/total number examined.
Number of the eggs with normal cleavage/total number examined.
Weak signal was observed in the noninjection side of the furrow. Volume of the injectants was adjusted to 4 nl.
