Table 2.
Results of coumarin's validation method
| Parameters | Systems | Results |
|---|---|---|
| Selectivity (Rf) | n-hexane:EA (8:2) (0.88) | 0.33 |
| CHCl3:MeOH (8:2)(4.33) | 0.89 | |
| EA:acetone: water (20:3:2) (4.85) | 0.92 | |
| Linearity (percentage CV) | Intraday | 1.1048 |
| 1.3345 | ||
| 0.9187 | ||
| Interday | 1.5936 | |
| 1.3190 | ||
| 1.3192 | ||
| Accuracy and precision, mean±SD LOD dan LOQ | Infusion | 100.70±1.30 |
| Ethanolic extract | 100.53±0.32 | |
| Y=4209.345 + 15.67116x | ||
| R2 = 0.99541 | ||
| LOD = 52.74 µg/mL | ||
| LOQ = 175.81 µg/mL | ||
LOD: Limit of detection, LOQ: Limit of quantification, SD: Standard deviation, CV: Coefficient of variation