Figure 1.

Genetic inhibition of MNK1 partially attenuates facial hypersensitivity and hyperalgesic priming caused by dural IL-6. (A) Female and male WT or MNK1 KO mice were administered 5 µl of the proinflammatory cytokine, IL-6 (0.1 ng), or vehicle onto their dura mater and tested for acute facial hypersensitivity (B) and grimace measures (C). Following resolution of acute allodynia, all mice were tested for hyperalgesic priming by administering 5 µl of SIF (pH = 7.0) and tested again. WT mice were observed to have significantly reduced withdrawal thresholds and increased grimacing compared to MNK1 KO mice, in which these effects were partially attenuated. Likewise, contrary to WT mice, MNK1 KO mice did not prime to dural pH 7.0. No sex differences were observed. Comparisons were made via two-way ANOVA followed by Bonferroni post hoc analysis. Significance between WT/IL-6 and MNK1 KO/IL-6 groups (denoted by asterisk) and between MNK1 KO/Veh and MNK1KO/IL-6 groups (denoted by delta symbol) is shown. n ≥ 6 for all groups; *P ≤ 0.05; **^,ΔΔP ≤ 0.01; ^ΔΔΔP ≤ 0.001; ****^,ΔΔΔΔP ≤ 0.0001.