Fig. 2.

Time depended effect of hypoxia on RGCs viability. Retinas were extracted and cultured as organotypic culture for different periods of times. Viability of RGCs was evaluated by staining for Brn3a. Repre-sentative pictures of retinas stained for Brn3a at (A) T0, (B) T24 hours, (C) T48 hours, (D) T72 hours. (E) Summary of time-dependent effect of hypoxic conditions on viability of RGCs. Bar=100 μm. Data are represented as mean±SEM. The experiment was conducted on 2 to 5 retinas per time point.