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. 2023 May 29;14:3092. doi: 10.1038/s41467-023-38663-7

Fig. 4. Fame is involved in the excretion of proteins and participates in the scaling of inner organs under adverse conditions such as nutrient deficiency.

Fig. 4

a (i) Violin plots showing the differences in weight between male wildtype and Fame knockout (KO) animals on an FVB/Ant background. Mean ± SEM and n for each group: WT (27.26 ± 0.3204 n = 4), KO (25.24 ± 0.1775 n = 4), p-value WT vs KO = 0.0015. ii Comparison of mean lean body mass. Mean ± SEM and n: WT (20.75 ± 0.3303 n = 4) KO (22.50 ± 0.5986 n = 4), p-value WT vs KO = 0.0436. (iii) Comparison of serum ferritin levels. Mean ± SEM and n: WT (3817 ± 548.6 n = 4) KO (2238 ± 295.5 n = 7), p-value WT vs KO = 0.0206. (iv) Comparison of urine albumin to creatinine ratio. Mean ± SEM and n: WT (0.07496 ± 0.007053 n = 11) KO (0.1983 ± 0.04809 n = 11), p-value Mann-Whitney test WT vs KO < 0.0001. b Micro-CT images of P0 pups with control and low iron diet, containing 178.58 mg iron/kg or 5.16 mg iron/kg, respectively. The kidney (red), interscapular brown adipose tissue (IBAT) (yellow), liver (green) and adrenal glands (orange) are segmented using 3D Visualization software and superimposed onto the pups. c–f Violin plots comparing inner organ scaling amongst P0 wildtype and knockout pups on different diets. c Kidney volume. Mean ± SEM and n: WT control (2.261 ± 0.2301, n = 8), KO control (1.401 ± 0.1354, n = 8), p-value WT control vs KO control = 0.0062, WT low iron (1.120 ± 0.0973, n = 8), KO low iron (1.642 ± 0.2669, n = 6). d IBAT volume. Mean ± SEM and n: WT control (8.44 ± 1.299, n = 4), KO control (4.955 ± 0.1169, n = 4), p-value WT control vs KO control = 0.037, WT low iron (4.210 ± 0.8292, n = 4), KO low iron (4.897 ± 0.9432, n = 3). e Liver volume. Mean ± SEM and n: WT control (29.94 ± 2.959, n = 4), KO control (19.90 ± 1.988, n = 4), p-value WT control vs KO control = 0.0304, WT low iron (17.72 ± 2.049, n = 4), KO low iron (16.72 ± 2.632, n = 3). f Adrenal gland volume. Mean ± SEM and n: WT control (0.095 ± 0.005345, n = 8), KO control (0.06125 ± 0.002950, n = 8), p-value WT control vs KO control < 0.0001, WT low iron (0.06250 ± 0.00491, n = 8), KO low iron (0.07667 ± 0.004944, n = 6). Source data are provided as a Source Data file.