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. 2023 May 29;14:3092. doi: 10.1038/s41467-023-38663-7

Fig. 6. Knock out of Fame influences metabolic parameters and activity.

Fig. 6

a Graphical representation of the performed metabolic cage and magnetic resonance imaging experiments. Measured parameters are listed. b (i-iv) Metabolic cage experiments using 75-day old male mice. Wildtype and Fame KO animals on FVB/Ant background are compared. 12-hour light (day) and dark (night) periods and 24-hour averages are shown. (i) Food intake normalized to body weight. Mean ± SEM and n for each group: WT day-time (3.780 ± 0.4648 n = 4), KO day-time (2.120 ± 0.3283 n = 4), p-value (day-time WT vs KO) = 0.0267, WT night-time (8.648 ± 0.3195 n = 4), KO night-time (8.785 ± 0.3498 n = 4), WT average (6.215 ± 0.3284 n = 4), KO average (5.453 ± 0.2056 n = 4). (ii) Energy expenditure, normalized by body weight. Mean ± SEM and n for each group: WT day-time (18.85 ± 0.1104 n = 4), KO day-time (17.46 ± 0.1311 n = 4), p-value (day-time WT vs KO) = 0.0002, WT night-time (22.77 ± 0.3909 n = 4), KO night-time (21.36 ± 0.1067 n = 4), p-value (night-time WT vs KO) = 0.0130, WT average (20.81 ± 0.2344 n = 4), KO average (19.41 ± 0.04249 n = 4), p-value (average WT vs KO) = 0.0011. (iii) Fine activity. Mean ± SEM and n for each group: WT day-time (152.0 ± 22.28 n = 4), KO day-time (111.6 ± 15.09 n = 4), WT night-time (444.2 ± 59.6 n = 4), KO night-time (307.4 ± 23.88 n = 4), p-value (night-time WT vs KO) = 0.0771, WT average (298.1 ± 32.48 n = 4), KO average (209.5 ± 17.0 n = 4), p-value Mann-Whitney test (average WT vs KO) = 0.0571. (iv) Z-activity. Mean ± SEM and n for each group: WT day-time (193.9 ± 61.34 n = 4), KO day-time (291.7 ± 83.16 n = 4), WT night-time (504.4 ± 120.1 n = 4), KO night-time (691.0 ± 176.1 n = 4), WT average (349.2 ± 81.28 n = 4), KO average (491.4 ± 122.9 n = 4). c (i-iv) Metabolic cage experiments using 70-day old male mice. Wildtype and Fame KO animals on C57Bl/6NCrl background are compared. 12-hour light (day) and dark (night) periods and 24-hour averages are shown. (i) Food intake normalized to body weight. Mean ± SEM and n for each group: WT day-time (1.996 ± 0.4721 n = 8), KO day-time (1.834 ± 0.3098 n = 10), WT night-time (11.92 ± 0.5476 n = 8), KO night-time (11.24 ± 0.3413 n = 10), WT average (6.958 ± 0.3622 n = 8), KO average (6.535 ± 0.2606 n = 10). (ii) Energy expenditure, normalized to body weight. Mean ± SEM and n for each group: WT day-time (11.13 ± 0.3114 n = 8), KO day-time (11.44 ± 0.1955 n = 10), WT night-time (18.04 ± 0.2678 n = 8), KO night-time (19.19 ± 0.5414 n = 10), WT average (14.59 ± 0.1513 n = 8), KO average (15.31 ± 0.3281 n = 10). (iii) Total activity (fine movements and beam breaks). Mean ± SEM and n for each group: WT day-time (256.6 ± 50.68 n = 8), KO day-time (214.6 ± 37.37 n = 10), WT night-time (691.9 ± 82.49 n = 8), KO night-time (584.6 ± 41.67 n = 10), WT average (474.4 ± 50.82 n = 8), KO average (399.5 ± 37.80 n = 10). (iv) Z-activity. Mean ± SEM and n for each group: WT day-time (36.63 ± 11.72 n = 8), KO day-time (11.90 ± 3.136 n = 10), p-value (day-time WT vs KO) = 0.0388, WT night-time (164.9 ± 24.29 n = 8), KO night-time (105.8 ± 12.59 n = 10), p-value (night-time WT vs KO) = 0.0359, WT average (100.8 ± 14.47 n = 8), KO average (58.80 ± 6.772 n = 10), p-value Mann-Whitney test (average WT vs KO) = 0.0059. Source data are provided as a Source Data file.