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. 2023 May 29;14:3092. doi: 10.1038/s41467-023-38663-7

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Analysis of FAME expression in various tumors and healthy tissues using RNAseq data obtained from The Cancer Genome Atlas (TGCA) Program. Mean ± SEM and n (tumor or healthy tissue samples): KIRP tumor (6.251 ± 0.0671 n = 289) kidney normal (5.916 ± 0.0301 n = 126) KIRC tumor (5.309 ± 0.0418 n = 530) CHOL normal (4.663 ± 0.1816 n = 9) KICH tumor (4.331 ± 0.1988 n = 65) CHOL tumor (4.056 ± 0.2020 n = 36) LIHC normal (4.199 ± 0.0688 n = 50) PAAD normal (2.872 ± 0.9882 n = 4) PAAD tumor (3.319 ± 0.0996 n = 178) LIHC tumor (3.255 ± 0.0623 n = 373) SARC normal (3.145 ± 3.145 n = 2) UCEC normal (1.295 ± 0.2736 n = 24). b FAME expression in kidney tumors (TCGA data) stratified by tumor grade (G)/stage (S). The expression is maintained in all tumor types derived from FAME expressing tissue. Mean ± SEM and n (tumor samples): KIRC S1 (5.437 ± 0.0472 n = 266) KIRC S2 (5.451 ± 0.1307 n = 57) KIRC S3 (5.236 ± 0.0894 n = 123) KIRC S4 (4.905 ± 0.1459 n = 81) KIRP S1 (6.455 ± 0.0737 n = 172) KIRP S2 (5.816 ± 0.3306 n = 22) KIRP S3 (5.811 ± 0.1692 n = 51) KIRP S4 (5.743 ± 0.3829 n = 15) KICH S1 (4.917 ± 0.1813 n = 20) KICH S2 (4.200 ± 0.3868 n = 25) KICH S3 (4.541 ± 0.2704 n = 14) KICH S4 (2.438 ± 0.8041 n = 6). c Proliferation analysis of HEK293T cells overexpressing FAME-EGFP or an EGFP control plasmid. EGFP⁺ cells were included in the analysis. Mean values of 5 independent cell clones per condition with positive error bars (SEM) are shown. Figure d as in c using human A549 cells. e Normalized RNA expression levels of FAME in A549 cells measured by qPCR. Technical replicates (n = 3) of two independent KO clones and one wildtype clone are shown. f Proliferation analysis of A549 clones in which FAME was knocked out using the CRISPR-Cas9 system. As control (WT), a FAME-sgRNA-transduced, but unedited clone in the locus of interest, was included. Mean values from 3 independent experiments with 2 replicates each per KO or WT clone (n = 6), with positive error bars (SEM), are shown. Source data are provided as a Source Data file. c, d, f Error bars are shown as positive SEM from the mean. Some bars are smaller than data symbols.